Human adult endothelial cell growth in culture
نویسندگان
چکیده
منابع مشابه
Human endothelial cells in culture.
Daily changes of cultured endothelial cells were observed by phase contrast microscopy, time-lapse microcinematography and electron microscopy. By light microscopy, the cultured cells are polygonal in shape and form monolayered clusters. However, they become elongated like fibroblasts between 10 and 14 days. Proliferation of the cultured cells by mitotic division could not be recognized by cine...
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BACKGROUND AND PURPOSE The cerebral cavernous malformation (CCM) is a common and frequently unrecognized cause of stroke and epilepsy. It consists of blood-filled caverns lined by endothelial cells (EC) and devoid of mature vessel wall structure. Cultured EC obtained from CCM may express phenotypic and genotypic alterations contributing to CCM pathogenesis. We report the first successful isolat...
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Regulator of calcineurin 3 (RCAN3) belongs to the human RCAN gene family, which also includes RCAN1 and RCAN2. All three members interact with and inhibit calcineurin. Based on this effect, several studies have demonstrated a role for RCAN1 and RCAN2 on inflammation, using human umbilical vein endothelial cells (HUVECs) as a model. RCAN1 and 2 are strongly induced by vascular endothelial growth...
متن کاملHuman Vascular Endothelial Cells in Culture
Human endothelial cells, obtained by collagenase treatment of term umbilical cord veins, were cultured using Medium 199 supplemented with 20% fetal calf serum. Small clusters of cells initially spread on plastic or glass, coalesced and grew to form confluent monolayers of polygonal cells by 7 days. Cells in primary and subcultures were identified as endothelium by the presence of Weibel-Palade ...
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ژورنال
عنوان ژورنال: Journal of Vascular Surgery
سال: 1984
ISSN: 0741-5214
DOI: 10.1016/0741-5214(84)90006-5